p drp  (Cell Signaling Technology Inc)

Journal: Endocrinology and Metabolism

Article Title: Irisin Attenuates Hepatic Stellate Cell Activation and Liver Fibrosis in Bile Duct Ligation Mice Model and Improves Mitochondrial Dysfunction

doi: 10.3803/EnM.2024.1984

Figure Lengend Snippet: Irisin attenuated transforming growth factor-β1 (TGF-β1)-induced mitochondrial fission in hepatic stellate cells (HSCs). LX-2 cells were treated with 5 ng/mL TGF-β1 and co-treated with 10 or 20 nM irisin for 24 hours. (A) Mitochondrial fission morphology was detected using transmission electron microscopy. Black arrows indicate mitochondria. (B) Western blot analysis of fission protein 1 (Fis1), phosphodynamin-related protein 1 (p-DRP1), phospho-mitochondrial fission factor (p-MFF), total DRP1, total MFF, optic atrophy 1 (OPA1), mitofusin 1 (MFN1), and mitofusin 2 (MFN2) expression in LX-2 cells. The quantitation of band intensities in Western blot images was calculated using ImageJ (National Institute of Health). The protein levels were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression. All data are presented as mean±standard error of mean ( n ≥3). a P <0.05, b P <0.01 vs. control.

Article Snippet: Primary antibodies included α-smooth muscle actin (α-SMA), superoxide dismutase 2 (SOD2), acetyl SOD2-K68 from Abcam (Cambridge, England); collagen type I (CO1A1) from Sigma-Aldrich (St. Louis, MO, USA); dynamin-related protein 1 (DRP1), phospho-DRP-1 (p-DRP1) (Serine 616), mitochondrial fission factor (MFF), phospho-MFF (p-MFF), sirtuin 3 (SIRT3), mitochondrial transcription factor A (TFAM), p-SMAD2, SMAD2, p-SMAD3, and SMAD3 from Cell Signaling Technology (Richmond, CA, USA); p-DRP1 (Serine 616) from Invitrogen (Waltham, MA, USA); fission protein 1 (Fis1) and succinate dehydrogenase subunit A (SDHA) from Santa Cruz Biotechnology (Dallas, TX, USA); and glyceraldehyde 3-phosphate dehydrogenase from GeneTex (Irvine, CA, USA).

Techniques: Transmission Assay, Electron Microscopy, Western Blot, Expressing, Quantitation Assay, Control

Journal: Endocrinology and Metabolism

Article Title: Irisin Attenuates Hepatic Stellate Cell Activation and Liver Fibrosis in Bile Duct Ligation Mice Model and Improves Mitochondrial Dysfunction

doi: 10.3803/EnM.2024.1984

Figure Lengend Snippet: Irisin recovered oxidative stress and mitochondrial dysfunction in bile duct ligation (BDL) mice. Irisin reversed the oxidative stress in the liver, as illustrated by the markers of oxidative stress. (A) The protein expression of acetyl superoxide dismutase 2-K68 (AcSOD2-K68) and (B) malondialdehyde (MDA) level. (C) Western blot analysis of fission protein 1 (Fis1), phospho-dynamin-related protein 1 (p-DRP1), phospho-mitochondrial fission factor (p-MFF), sirtuin 3 (SIRT3), mitochondrial transcription factor A (TFAM), and succinate dehydrogenase subunit A (SDHA) expression in liver. The quantitation of band intensities in Western blot images was calculated using ImageJ (National Institute of Health). The protein levels were normalized to glyceraldehyde 3-phosphate dehydrogenase (GAPDH) expression. All data are presented as mean±standard error of mean ( n ≥3). SOD2, superoxide dismutase 2; NS, no significance. a P <0.05, b P <0.01 vs. sham group; c P <0.05, d P <0.01 vs. BDL group.

Article Snippet: Primary antibodies included α-smooth muscle actin (α-SMA), superoxide dismutase 2 (SOD2), acetyl SOD2-K68 from Abcam (Cambridge, England); collagen type I (CO1A1) from Sigma-Aldrich (St. Louis, MO, USA); dynamin-related protein 1 (DRP1), phospho-DRP-1 (p-DRP1) (Serine 616), mitochondrial fission factor (MFF), phospho-MFF (p-MFF), sirtuin 3 (SIRT3), mitochondrial transcription factor A (TFAM), p-SMAD2, SMAD2, p-SMAD3, and SMAD3 from Cell Signaling Technology (Richmond, CA, USA); p-DRP1 (Serine 616) from Invitrogen (Waltham, MA, USA); fission protein 1 (Fis1) and succinate dehydrogenase subunit A (SDHA) from Santa Cruz Biotechnology (Dallas, TX, USA); and glyceraldehyde 3-phosphate dehydrogenase from GeneTex (Irvine, CA, USA).

Techniques: Ligation, Expressing, Western Blot, Quantitation Assay